Journal: Nature Communications

Article Title: From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator

doi: 10.1038/s41467-024-51626-w

Figure Lengend Snippet: E. coli bacteria host a synthetic optogenetic oscillator. Growing colonies form concentric rings of the fluorescent reporters. The oscillations can either be free-running (constant light) or forced (cycles of light and dark). Resonance occurs when the frequency of the external forcing matches with the frequency of the free-running oscillator. Subharmonic resonance occurs when the cells oscillate at a frequency that is a fraction of the driving frequency, whereas superharmonic resonance occurs when a system oscillates at a frequency that is a multiple of the driving frequency. Resonance phenomena are characterised by an increase in the amplitude of oscillations. Changes of the external forcing frequency can lead to bifurcation, where the period of oscillation is doubled (hence period-doubling) and a pattern of two peaks per every oscillation appears (period-2). Successive period-doubling events lead to period- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N , with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N peaks repeating per oscillation. Further period-doubling can drive the system to chaos, where small variations between the initial parameters will lead to huge changes in the course of oscillations. The lack of repetitive pattern in the chaotic system makes it impossible to predict the trajectory of oscillations. In this scenario cells are expected to quickly desynchronise, precluding the visualisation of ring patterns. Illustration of colonies with oscillations was inspired by .

Article Snippet: To generate the bacterial chassis, we deleted the clxCP gene from the E. coli MK01 strain , which was a kind gift of Sander Tans (Addgene #195090).

Techniques: Bacteria

Journal: Nature Communications

Article Title: From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator

doi: 10.1038/s41467-024-51626-w

Figure Lengend Snippet: Primers list

Article Snippet: To generate the bacterial chassis, we deleted the clxCP gene from the E. coli MK01 strain , which was a kind gift of Sander Tans (Addgene #195090).

Techniques: Construct, Sequencing

Journal: Stem Cells International

Article Title: Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease

doi: 10.1155/2020/4085617

Figure Lengend Snippet: Basic characteristics of the cannulas.

Article Snippet: MK01 was modified from the Mizuho cannula and has the following characteristics ( ): it is made of 100% stainless steel, the outer diameter of the cannula is 1.5 mm, and the internal bore diameter is 300 μ m, which enables direct attachment to the Leksell stereotactic arc system (Model G, Elekta, Sweden).

Techniques: Injection

Journal: Stem Cells International

Article Title: Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease

doi: 10.1155/2020/4085617

Figure Lengend Snippet: Photograph of the MK01 cannula and of the shape of the open orifice. The distal side of the opening orifice is curved just proximal to the equator line of the spherical tip so that the opening orifice does not appear from the inserting trajectory view.

Article Snippet: MK01 was modified from the Mizuho cannula and has the following characteristics ( ): it is made of 100% stainless steel, the outer diameter of the cannula is 1.5 mm, and the internal bore diameter is 300 μ m, which enables direct attachment to the Leksell stereotactic arc system (Model G, Elekta, Sweden).

Techniques:

Journal: Stem Cells International

Article Title: Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease

doi: 10.1155/2020/4085617

Figure Lengend Snippet: Photographs of compressing the cervical artery using the Pittsburg cannula (a) and of massive hemorrhage after arterial vessel penetration (b). Graph showing the force needed to penetrate the abdominal arterial wall with each cannula (c). The Pittsburg cannula required a significantly smaller force to penetrate the artery compared with the Mizuho and MK01 cannulas.

Article Snippet: MK01 was modified from the Mizuho cannula and has the following characteristics ( ): it is made of 100% stainless steel, the outer diameter of the cannula is 1.5 mm, and the internal bore diameter is 300 μ m, which enables direct attachment to the Leksell stereotactic arc system (Model G, Elekta, Sweden).

Techniques:

Journal: Stem Cells International

Article Title: Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease

doi: 10.1155/2020/4085617

Figure Lengend Snippet: Representative figures of the jet flow with each cannula (a, arrow) and the distance reached by saline (b) ( †,§ p < 0.05 vs. MK01). The force (N) needed to cause vertical jet flow (fountain) (c) ( ∗ p < 0.05).

Article Snippet: MK01 was modified from the Mizuho cannula and has the following characteristics ( ): it is made of 100% stainless steel, the outer diameter of the cannula is 1.5 mm, and the internal bore diameter is 300 μ m, which enables direct attachment to the Leksell stereotactic arc system (Model G, Elekta, Sweden).

Techniques:

Journal: Stem Cells International

Article Title: Evaluation of Novel Stereotactic Cannula for Stem Cell Transplantation against Central Nervous System Disease

doi: 10.1155/2020/4085617

Figure Lengend Snippet: Colored saline injected in the gel imitating the brain. The Pittsburg and Mizuho needles showed jet flow prominence (arrow) at 2000 μ L/min (arrow), while MK01 did not cause protuberance.

Article Snippet: MK01 was modified from the Mizuho cannula and has the following characteristics ( ): it is made of 100% stainless steel, the outer diameter of the cannula is 1.5 mm, and the internal bore diameter is 300 μ m, which enables direct attachment to the Leksell stereotactic arc system (Model G, Elekta, Sweden).

Techniques: Injection

Journal: Cell host & microbe

Article Title: Klebsiella oxytoca causes colonization resistance against multidrug-resistant K. pneumoniae in the gut via cooperative carbohydrate competition.

doi: 10.1016/j.chom.2021.09.003

Figure Lengend Snippet: Figure 3. K. oxytoca MK01 promotes decolonization of K. pneumoniae in humanized microbiota mice (A–C) GF animals received fecal transplants (FT) from 4 different SUS donors from the MikroKids and MikroResist cohort (n = 2 mice per donor). After 14 days, mice were treated with amp for 6 consecutive days, and one mouse per donor was precolonized with 108 CFUs K. oxytoca MK01 for 22 weeks before colonization. At day 3 of precolonization all mice received a second cycle of amp and were colonized with 108 CFUs K. pneumoniae MDR1 at day 0. Colonization kinetics (B) and fecal burden on day 21 (C) of K. pneumoniae MDR1. p values indicated represent a paired test *p < 0.05 between SUS donor mice and SUS donor mice treated with MK01. See also Figure S3.

Article Snippet: Strains used for precolonization were obtained Cell Host & Microbe 29, 1663–1679.e1–e7, November 10, 2021 e4 from the human cohorts including K. oxytoca isolates (MK01-MK19, MR01-MR04) and a commensal E. coli isolate (EC103) or purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) (K. oxytoca DSM5175/ATCC13182).

Techniques: